Reporter

Part:BBa_K1204013:Design

Designed by: Matthew Baron   Group: iGEM13_Duke   (2013-09-16)


TEF1pr-BS1x-16A


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 521
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 176


Design Notes

We had to consider the manner in which iTALs and gRNA/dCas9 complex bind in designing our sequence.


Source

The binding site was synthetically designed using the binding rules for iTALs and gRNA/dCas9 complex and ordered as a gene block from IDT. The TEF1 promoter is a strong constitutive promoter in yeast that we got from another plasmid in the lab.

References